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COLLEGE OF MICRONESIA

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P.O Box 1179 Kolonia,Pohnpei FM 96941
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Kosrae Research

Integrated research and outreach on in vitro multiplication, sustainable, organic and climate-smart commercial cultivation of black pepper

January 21, 2018

KOSRAE, FSM. Dr. Virendra M. Verma, Researcher and Extension Specialist, Kosrae Agricultural Experiment Station.

Black pepper (Piper nigrum L.) a flowering vine of Piperaceae family, is valued for its dried berries called peppercorns, which are used as a spice and for medicinal purposes. Native to the humid jungles of the Malabar Coast of Southwestern India, the plant is cultivated in the tropics worldwide. In Micronesia, it is gaining commercial importance as an important cash crop because of premium price. Traditionally, the trunks of two cultivars of large native tree fern (Cyathea nigricans) are used as supports for black pepper cultivation. However, short life-span of these tree ferns along with the rapid decline in their population due to increasing use of tree trunks for construction, is becoming a limitation for commercial black pepper cultivation in the region.

Therefore, to promote sustainable black pepper cultivation in the region, an integrated research, outreach and education project entitled, ‘Black pepper micropropagation for elite seedling production: Comparison of local practices and commercial cultivation methods’ was initiated by the Project Director Dr. Virendra M. Verma. This project is approved and funded by the United States Department of Agriculture, National Institute of Food and Agriculture (USDA-NIFA) for Micronesia. This project is of great significance as it is specifically designed to develop black pepper micropropagation and nursery management systems to produce and ensure the year-round availability of identical, disease-free and high-quality planting material. The objectives of the project include: finding alternative supports to overcome the limitations caused due to shortage of tree ferns, determining appropriate fertilizer type and doses, along with the development and publication of a commercial black pepper cultivation guide appropriate for Micronesia.

This project is first of its kind not only in the Micronesian region but also in the Pacific region because it is integrating and employing multiple latest tools and technologies such as plant biotechnology, horticulture, microbiology, plant physiology and plant pathology for sustainable, climate-smart and organic commercial cultivation of black pepper. The project is utilizing plant biotechnological techniques such as in vitro cloning for uniform black pepper plantlet production, greenhouse acclimatization of cloned black pepper plantlets for elite, disease-free seedling production, automatic fertilizer injectors for uniform fertilizer application, and organic fertilizers to provide essential nutrients and maintain beneficial soil microorganisms along with appropriate site-specific and climate-smart horticultural, plant physiological and integrated pest management practices. The project activities also include educating local farmers in site-specific sustainable agricultural practices, and providing appropriate recommendations and training for sustainable, climate-smart and organic commercial black pepper production in the region.

Successful execution of project objectives has resulted in the development of a highly efficient and reliable in vitro cloning method for multiplication and production of elite, uniform and diseases-free black pepper plantlets, and acclimatization of hundreds of cloned plantlets into uniform and diseases-free seedlings of a locally preferred and commercially important black pepper cultivar Piper nigrum cv. Srilanka through appropriate nursery management systems.

Considering the extremely limited availability of traditional tree fern supports and their very short lifespan, non-living supports such as reinforced cement-concrete standards have been specifically designed and constructed at pilot site to support the vines of fully acclimatized black pepper plants in the field. In addition, raised beds, which ensure perfect water drainage, organic fertilizers for soil amendment along with organic mulching, and automatic fertilizer injectors which provide perfect nutrition, were used for the establishment and maintenance of black pepper plantations.

Research trials for commercial black pepper cultivation have been designed, and implemented at pilot site, and data collection is in progress for analysis. The outcomes of this analysis would be ultimately used for the development and publication of a commercial cultivation guide to specifically suit the needs of local farmers. The project is also conducting comparative research study of local black pepper cultivation practices and sustainable commercial cultivation methods. Pilot site is being developed and would be used as demonstration site to carry out research, outreach and education activities of the project to encourage and promote sustainable commercial black pepper production among local farmers in the region. Recommendations for sustainable black pepper production would be provided through training workshops, hands-on trainings, farm visits, and field days.

The overall aim of the project is to develop sustainable black pepper production systems in Micronesia by providing elite and disease-free seedlings, appropriate recommendations for commercial black pepper production such as sustainable, organic and climate-smart technology and developing skills and improving the agricultural knowledge of local farmers. Adoption of new practices such as micropropagation of black pepper for improved and enhanced productivity will ultimately help in reviving local black pepper industry. Furthermore, this project would provide opportunities for income generation and profitable self-employment to the participating farmers. In addition, the pilot site is serving as an excellent cultivation model to encourage other farmers and rural communities in successfully adopting the sustainable, climate-smart and organic commercial black pepper production practices. Enjoy these photos of this project!

In vitro multiplication of black pepper seedling.
Reinforced cement concrete standards or supports.
Soil amendments with organic fertilizers.
Amended soil filled around standards.
Raised beds for crop management and disease-free seedlings.
Establishment of black pepper plants in field.
Black pepper vine training.
Black pepper cultivation and best disease management practices.
Elevated beds for perfect water drainage.
Liquid fertilizer application through fertilizer injector.
Healthy and vigorous black pepper vines – One year old after planting.
Black pepper flowering.
Black pepper fruit setting.
Black pepper vine pruning.
Ready to harvest drupes of black pepper.
Harvested drupes of black pepper.
Close-up of harvested drupes of black pepper.
Processing of black peppercorns.
Close-up of processed black peppercorns.
Close-up of processed white peppercorns.

Filed Under: Kosrae Extension, Kosrae Research, News from land grants

In vitro mass-scale, disease-free pineapple seedling production: Research and multiple demonstrations on sustainable, climate-smart and organic commercial cultivation

March 29, 2017

KOSRAE, FSM. Dr. Virendra M. Verma, Researcher and Extension Specialist, Kosrae Agricultural Experiment Station.

The pineapple (Ananas comosus L. Merrill), a tropical plant with edible multiple fruit consisting of coalesced berries, is the most economically significant plant in the Bromeliaceae family.

Pineapples are delicious tropical fruit that have been celebrated for centuries for their distinct and unique taste and miraculous health benefits. The health and medicinal benefits of pineapple include the ability to improve respiratory, digestive and immune systems. Pineapple consumption cures coughs and colds, strengthens bones, improves oral health, boosts eye health, increases heart health and blood circulation, reduces inflammation, fights off infections and parasites, prevents cancer, and also helps in losing weight (Szalay, 2014).

‘Pineapple micropropagation and commercial cultivation to enhance productivity in Micronesia’, is an integrated research, outreach and education project approved by the United States Department of Agriculture, National Institute of Food and Agriculture (USDA-NIFA) for the Micronesian region. According to Dr. Virendra M. Verma, the project director of this project, enhancing pineapple production in the Federated States of Micronesia (FSM) and the Republic of the Marshall Islands (RMI) will help in overcoming the regional agricultural challenges primarily, the non-availability of disease-free and uniform seedlings, shortage of trained agricultural professionals, and limited acquaintance of farmers with sustainable, commercial cultivation practices. By providing a means of sustainable, climate-smart and organic commercial cultivation of pineapple, this project will create opportunities of income generation for local farmers, and will ultimately result in improved food self-sufficiency, and nutritional security in the region.

This project with multiple sites across the Micronesian region is first of its kind not only in the Micronesian region but also in the Pacific region because it is integrating and employing multiple latest tools and technologies such as plant biotechnology, horticulture, microbiology, plant physiology and plant pathology for sustainable, climate-smart and organic commercial cultivation of pineapple. The project is of great significance as it is specifically designed to optimize in vitro multiplication protocol and nursery management systems to produce elite and disease-free pineapple seedlings in bulk quantities in the Micronesian region. The project is utilizing plant biotechnological techniques of in vitro mass-scale cloning for uniform pineapple plantlet production, greenhouse acclimatization of cloned pineapple plantlets for mass-scale, disease-free seedling production, and organic fertilizers to provide essential nutrients and maintain beneficial soil microorganisms along with appropriate site-specific and climate-smart horticultural, plant physiological and integrated pest management practices. The project activities also include educating local farmers in site-specific sustainable agricultural practices, and providing appropriate recommendations and training for sustainable, climate-smart and organic commercial pineapple production in the region.

Successful implementation of this project has resulted in the development of a high efficiency and reliable, in vitro cloning method for mass multiplication and production of elite, uniform and diseases-free pineapple plantlets, and acclimatization of thousands of cloned plantlets into uniform and diseases-free seedlings of selected pineapple cultivars such as Ananas comosus cv. Kosraean and Ananas comosus cv. Hawaiian through appropriate nursery management systems. Preparation has been started for designing, implementing and conducting research trials for commercial pineapple cultivation at multiple sites as well as for collecting data for analysis, and the development and publication of a commercial pineapple cultivation guide to specifically suit the needs of local farmers. Recommendations for sustainable pineapple production are being provided through training workshops, hands-on trainings, farm visits, and field days. Three pilot sites in the Micronesian region, specifically in the Federated States of Micronesia (FSM) and Republic of Marshall Islands (RMI) are being developed as demonstration sites to carry out research, outreach and education activities of the project to encourage and promote sustainable commercial pineapple production among local farmers in the region.

This project is providing opportunities for income generation and profitable self-employment to the participating farmers. In addition, it is serving as an excellent example to encourage other farmers and rural communities in successfully adopting the sustainable, climate-smart and organic commercial pineapple production practices.

Szalay, J (2014) Pineapple: health benefits, risks & nutrition facts (Live science reference). Retrieved from http://www.livescience.com/45487-pineapple-nutrition.html

In vitro cultures of pineapple.

 

In vitro multiplication of pineapple.

 

Pineapple flowering (Ananas comosus cv. Kosraean).

 

Pineapple cultivar (Ananas comosus cv. Kosraean).

 

Pineapples (Ananas comosus cv. Kosraean).

 

Pineapple flowering (Ananas comosus cv. Hawaiian).

 

Pineapple (Ananas comosus cv. Hawaiian).

 

Pineapples (Ananas comosus cv. Hawaiian).

 

Pineapple flowering (Ananas comosus cv. Hawaiian thorn-less).

 

Pineapple (Ananas comosus cv. Hawaiian thorn-less).

 

Pineapple (Ananas comosus cv. Hawaiian thorn-less).

 

Pineapple demonstration plot.

 

Pineapple research trials.

 

Pineapple cultivar Hawaiian thorn-less research trials.

 

Pineapple cultivar Hawaiian research trials.

 

Pineapple cultivar Kosraean research trials.

Filed Under: Kosrae Extension, Kosrae Research, News from land grants Tagged With: Kosrae, Pineapple

Highly efficient in vitro multiplication of commercially important black pepper cultivar, and sustainable commercial cultivation in Micronesia

February 26, 2017

KOSRAE, FSM. Dr. Virendra M. Verma, Researcher and Extension Specialist, Kosrae Agricultural Experiment Station.

Black Pepper Micropropagation for Elite Seedling Production: Comparison of Local Practices and Commercial Cultivation Methods, is a multi-state research project approved by the United States Department of Agriculture, National Institute of Food and Agriculture (USDA-NIFA) for the Micronesian region. The project director of this project, Dr. Virendra M. Verma, shared that this project involves multiplication of elite and disease-free black pepper seedlings, and a comparative research study of local practices and commercial cultivation methods. The project aims towards developing sustainable black pepper production systems in Micronesia by providing elite and disease-free seedlings, appropriate recommendations for commercial black pepper production, and developing skills and improving the agricultural knowledge of local farmers. Adoption of new practices such as micropropagation of black pepper for improved and enhanced productivity will ultimately help in reviving local black pepper industry.

This project is of great significance as it is specifically designed to develop black pepper micropropagation and nursery management systems to produce elite seedlings in bulk quantities to ensure the year-round availability of identical, disease-free and high quality planting material, find alternatives for tree fern supports, determine appropriate fertilizer type and doses, along with the development and publication of a commercial black pepper cultivation guide appropriate for Micronesia.

Black pepper (Piper nigrum L.) is a flowering vine of Piperaceae family, which is often cultivated for its berries that are usually dried and used as a spice. Native to the humid jungles of the Malabar Coast of Southwestern India, black pepper is currently cultivated worldwide in the tropics. In Micronesia, it is gaining commercial importance as an important cash crop. Traditionally, the trunks of large native tree fern (Cyathea nigricans), serve as supports for commercial black pepper cultivation. Two cultivars of the tree ferns are traditionally recognized, the preferred cultivar produces juice which stains red, and the other produces a greyish juice. However, the population of these tree ferns in the region is rapidly declining due to use of tree trunks for construction of traditional houses, and their short life-span.

In this project, an economically feasible, efficient, rapid and reproducible in vitro multiplication protocol was developed for a locally preferred and commercially important black pepper cultivar (Piper nigrum cv. Srilanka). Murashige and Skoog, 1962 medium (MS) was used throughout the study. The cultures were initiated by inoculating shoot meristems as explants on MS medium augmented with various concentrations and combinations of cytokinins and auxins. To prevent the browning of explants, 100mgL-1 ascorbic acid was added to all media. The best culture initiation was observed on the MS medium augmented with 5µM 6-benzylaminopurine (BAP). For further growth and subsequent multiplication, the established cultures were transferred on MS medium augmented with 1µM BAP. The number of multiple shoots produced from each explant after two subcultures varied from 8 to 20. A 16-h photoperiod with a temperature of 24oC day and night, light intensity of 40µmol m-2 s-1, and 60% relative humidity were maintained for multiplication. Developed multiple shoots were transferred on MS medium augmented with 2µM indole-3-acetic acid for rooting. Complete plantlets were transferred to soil: vermiculate (1:1, v/v) mixture in 72-cell trays and acclimatized with 68% survival rate in 10 weeks. Fully acclimatized plants were planted in the field.

Considering the extremely limited availability of traditional tree fern supports and their very short lifespan, non-living supports such as reinforced cement-concrete standards have been specifically designed and constructed at the project’s pilot site to support the vines of fully acclimatized black pepper plants in the field. In addition, raised beds to ensure perfect water drainage, organic fertilizers for soil amendment along with organic mulching, and automatic fertilizer injectors to provide perfect nutrition, were used for the establishment and maintenance of black pepper plantations.

The successful implementation of this project has resulted in the development of a high efficiency in vitro multiplication method for the mass production of elite, diseases-free seedlings, and the acclimatization of hundreds of tissue-culture raised plants of selected black pepper cultivar through appropriate nursery management systems. Preparation has been done for designing, implementing and conducting research trials for commercial black pepper cultivation at multiple sites. The development and publication of a commercial black pepper cultivation guide to specifically suit the needs of local farmers, is also underway. To carry out research, extension, and education activities of the project, two sites in Micronesia will be developed as demonstration sites. At these sites appropriate recommendations will be provided for sustainable black pepper production through training workshops, hands-on trainings, farm visits, and field days, which will encourage and promote sustainable commercial black pepper production among local farmers in the region.

To disseminate the scientific and technical knowledge gained from the project further, Dr. Verma has participated as a prominent speaker and resource person in various conferences. His contributions specifically informed abiotic and biotic stress management for sustainable pepper production in Micronesia, and served as a guiding source for other black pepper producing countries.

In-vitro cultures of black pepper.
Acclimatized black pepper plants in nursery.
Reinforced cement concrete standards.
Soil amendments with organic fertilizers around reinforced cement concrete standards.
Soil amendments with organic fertilizers.
Raised bed for perfect water drainage and in vitro multiplied disease-free seedlings.
Establishment of black pepper plants in field.
Dr. Verma contributing valuable scientific and technical knowledge with other black pepper producing countries.
Honoring Dr. Virendra M. Verma as main technical and prominent resource person.

Filed Under: Kosrae Extension, Kosrae Research, News from land grants Tagged With: Black pepper, Kosrae

Development of Salt Tolerant Sweet Potato (Ipomoea batatas (L.) Lam.) through Tissue Culture

November 8, 2016

KOSRAE, FSM. Dr. Virendra M. Verma, Researcher and Extension Specialist, Kosrae Agricultural Experiment Station, Kosrae.

Salinity and drought still remain the major abiotic stresses, which limit and pose a threat to agricultural production in many parts of the world including Micronesia. Dr. Virendra M. Verma’s research project entitled, “In Vitro Selection for Salt Tolerance in Sweet Potato,” is one effort to counteract these abiotic challenges, and contribute in sustainable agricultural production in Micronesian region. Approved by the United States Department of Agriculture, National Institute of Food and Agriculture (USDA-NIFA), this project focuses on developing salt tolerant varieties of Sweet Potato (Ipomoea batatas (L.) Lam.). The project director of this project, Dr. Verma, shared that both plant biotechnology and breeding methods were employed for the selection, assessment, and development of salt tolerant Sweet Potato varieties. Sweet Potato, a common food crop in Micronesia, was selected as the focus crop for this project because of its ‘typhoon-proof’ nature. Even after a typhoon(s), the storage roots of sweet potato remain protected under the ground, and can be used to reestablish the crop. Considering the local climatic conditions and socio-cultural demand for Sweet Potato in Micronesia, the development of salt tolerant varieties of the crop in the region, is of great significance.

Spread over a million square miles of the Western Pacific Ocean with a total land area of only about 271 square miles, the Federated States of Micronesia is made up of 607 small islands. Agriculture is an important industry that could greatly help in the economic development and growth through bringing food self-sufficiency in the region. However, current agricultural programs in Micronesia, are mostly at subsistence level, and economic development is largely dependent on the outside world. Serious damage caused by natural calamities such as wave surges, salt water flooding, and drought, continually pose challenges for the local farmers. Moreover, lack of technical know-how and changing life style along with the food habits of islanders, leading to an increase in the consumption of imported foodstuff; have led to an overall decline in local agricultural production in the region.

With the effects of global climate change and the increasing occurrence of natural disasters, farmers must be made aware of, and trained to adopt preventative measures to minimize impact of these disasters on their agricultural operations. Of all the cultivated root and tuber crops, sweet potato is probably the best at adapting quickly to new conditions. But still, its growth and development can be affected by adverse environmental conditions such as frequent wave surges and salt spray. Salinity, an abiotic stress that combines elements of water deficiency and sodium toxicity resulting in lost crop yield and arable land, is among the most serious and widespread of agricultural problems on the Micronesian islands. Hence, the focus of this project is to develop salt tolerant varieties of sweet potato.

Sweet potato ranks seventh in the world among food crops with respect to annual production, and it is the fifth most important food crop in developing countries. It is a highly functional low calorie food that contains various key nutrients. Every sweet potato contains high levels of starch, along with abundant vitamins (B-carotene, B1, C and E), minerals (Potassium) and dietary fiber (such as cellulose and pectin). To meet the ever-increasing demands of this nutritionally-rich and culturally-preferred food crop, there is crucial need to increase its agricultural production.

The cultivation of sweet potato is becoming more and more important with each passing day as the crop has great potential as food product. It needs a few inputs and has potentially high yields, which make it an inexpensive food. In addition, it is well suited for processing into food products for people and animals alike. Considered as the crucial staple crops for ensuring food, nutritional, and economic security, sweet potatoes are placed on high agricultural priority in the Micronesian region. However, the limitations in availability of salt-tolerant germplasm, and disease-free and elite seedlings, are a major bottleneck in sweet potato production. The ability to successfully grow sweet potato for sustenance in Micronesia depends on availability of salt tolerant varieties and on skillful use of appropriate cultivation techniques.

The study undertaken in Dr. Verma’s this research project, involved the assessment and development of salt tolerance in sweet potato through in vitro, greenhouse, and field selection of tissue-culture raised plants. To establish aseptic cultures of collected sweet potato germplasm, various in vitro experiments were performed. Different concentrations of sodium chloride were used for in vitro selection of salt tolerant germplasm, which was further evaluated for salt tolerance in the greenhouse., In vitro selected germplasm of salt tolerant sweet potato was finally field evaluated at eight sites in four replications. Results based on various physiological and morphological parameters collected during this research indicate that the developed salt tolerant varieties of sweet potato performed very well at coastal sites where salt spray due to high wave surges is very frequent. The findings of this research are being disseminated locally and globally through presentations in national and international conferences. To provide local farmers and producers with guidelines for cultivating sweet potato, an extension publication in form of a book entitled, “Sweet Potato Cultivation Guide,” has been published.

Invitro cultures of salt-tolerant sweet potato.
Invitro cultures of salt-tolerant sweet potato.
salt-tolerant-sweet-potato-in-field-for-evaluation
Salt-tolerant sweet potato-in-field for evaluation.

The cultivation of developed salt tolerant sweet potato varieties in Micronesia through this research project will lead to successful production of sweet potato in the region, and contribute in sustaining traditional socio-cultural life of the Micronesian people, and thereby, play a critical role in ensuring food and nutritional security of the region.  Examples of raw and cooked test varieties are below.  Recently, many countries have embraced sweet potato as a substitute for imported carbohydrates and for attaining national food security goals. The crop is also gaining importance as a key income earner for many small-scale producers, and a foreign exchange earner for many countries. As such, there is a growing global demand for sweet potato. It is our hope that the outcomes of this research will provide current and potential farmers and producers with practical information that will assist in developing sustainable crop production systems, and increasing productivity of sweet potato in Micronesia and beyond.

Selected salt-tolerant-sweet potato germplasm - cultivar 1.
Selected salt-tolerant-sweet potato germplasm – cultivar 1.
steamed-salt-tolerant-sweet-potato-germplasm-for-taste-evaluation-1
Steamed salt-tolerant-sweet potato germplasm – cultivar 1.

 

Selected salt-tolerant-sweet potato germplasm - cultivar 2.
Selected salt-tolerant-sweet potato germplasm – cultivar 2.
steamed-salt-tolerant-sweet-potato-germplasm-for-taste-evaluation-2
Steamed salt-tolerant-sweet potato germplasm – cultivar 2.

 

Selected salt-tolerant-sweet potato germplasm - cultivar 3.
Selected salt-tolerant-sweet potato germplasm – cultivar 3.
steamed-salt-tolerant-sweet-potato-germplasm-for-taste-evaluation-3
Steamed salt-tolerant-sweet potato germplasm – cultivar 3.

Filed Under: Kosrae Extension, Kosrae Research, News from land grants

Development of Salt tolerant giant swamp taro (Cyrtosperma merkusii (Hassk.) Schott) and soft taro (Colocasia esculenta (L.) Schott) through tissue culture

May 6, 2016

KOSRAE, FSM. Dr. Virendra M. Verma, Researcher and Extension Specialist, Kosrae Agricultural Experiment Station.

Salinity and drought still remain the major abiotic stresses that limit as well as pose a threat to agricultural production in many parts of the world including Micronesia. The research project entitled, “In Vitro Selection for Salt Tolerance in Taro,” focused on developing salt tolerant varieties of giant swamp taro (Cyrtosperma merkusii (Hassk.) Schott), and soft taro (Colocasia esculenta (L.) Schott). Approved by the United States Department of Agriculture, National Institute of Food and Agriculture (USDA-NIFA), this project incorporated both plant biotechnology and breeding methods for the selection, assessment, and development of salt tolerant taro varieties. The project director of this project, Dr. Virendra M. Verma, shared that since taro is grown in lowland swamp areas across Micronesia, the crop is especially vulnerable to damage from saltwater. Considering the traditional and cultural importance given to giant swamp taro and soft taro as the most important staple food crops in the Micronesia region, the availability of salt tolerant taro varieties through this research project, is of great significance.

Giant swamp taro and soft taro, members of the family Araceae, are ancient crops that are grown throughout the humid tropics for their edible corms, leaves and petioles as well as for traditional uses. Nutritionally, taro is rich in fiber, calcium, potassium, iron, vitamin A, vitamin B1, vitamin B2, and vitamin C. The corms of taro are superior to potatoes in nutritional value, containing a higher proportion of proteins, calcium and phosphorus. The vitamin B content in taro corms is equivalent to that of cabbage and twice that of potato. Taro also contains greater amounts of vitamin B-complex than whole milk. In corms, the main bulk starch is present in very fine grains which make them easily digestible. The corms can be boiled, roasted, fried as chips or cooked in curries. The young leaves and petioles of soft taro are also used as food and cooked like any other green vegetable. The cooked leaves of soft taro have the same nutritional value of spinach.

Salinity, an abiotic stress that combines elements of water deficiency and sodium toxicity resulting in lost crop yield and arable land, is among the most serious and widespread of agricultural problems on the Micronesian islands. Giant swamp taro and soft taro are among the most important staple food crops in the region. Cultivated for local consumption as well as for export, these crops contribute significantly to the regional socio-economic growth and provide livelihood to almost all island people. Considered as the crucial staple crops for ensuring food, nutritional, and economic security, taros are placed on high agricultural priority in the region. However, the limitations in availability of salt-tolerant germplasm, and disease-free and elite seedlings, are a major bottleneck in taro production. Therefore, the efforts to develop salt-tolerant plants are of immense importance to increase crop productivity. In recent years, tissue culture based in vitro selection has emerged as a feasible and cost-effective tool for developing and/or screening salt tolerant germplasm.

The research study undertaken in Dr. Verma’s project involved assessment and development of salt tolerance in giant swamp taro and soft taro through in vitro, greenhouse, and field selection of tissue-culture raised plants. To establish aseptic cultures of collected taro germplasm, various in vitro experiments were performed. Different concentrations of sodium chloride were used for in vitro selection of salt tolerant germplasm. This in vitro selected germplasm was further evaluated for salt tolerance in the greenhouse, and was finally field evaluated at eight sites in four replications. In vitro selected germplasm of salt tolerant taros performed very well at coastal sites. Results based on various physiological and morphological parameters collected during this research are presented in international conferences, and a manuscript is under preparation to be published in peer-reviewed and reputed scientific journals.

The cultivation of the developed salt tolerant taro varieties in the Micronesian region through this research project would not only lead to the successful production of taro but would also contribute for the food and nutritional security of the region, and thereby, play a critical role in the preservation of traditional socio-cultural life of the Micronesian people.

In vitro cultures of salt tolerant soft taro.
In vitro cultures of salt tolerant soft taro.
Salt tolerant soft taros in field for evaluation.
Salt tolerant soft taros in field for evaluation.

 

In vitro cultures of salt tolerant giant swamp taro.
In vitro cultures of salt tolerant giant swamp taro.
Salt tolerant giant swamp taros in field for evaluation.
Salt tolerant giant swamp taros in field for evaluation.

Filed Under: Kosrae Extension, Kosrae Research, News from land grants Tagged With: Kosrae, taro, tissue culture

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