Pineapple

KOSRAE, FSM. Dr. Virendra M. Verma, Researcher and Extension Specialist, Kosrae Agricultural Experiment Station

Pineapple Micropropagation and Commercial Cultivation to Enhance Productivity in Micronesia, is a multi-state research project approved by the United States Department of Agriculture, National Institute of Food and Agriculture (USDA-NIFA) for the Micronesian region. The project director of this project, Dr. Virendra M. Verma, shared that this project focuses on enhancing pineapple production in the Federated States of Micronesia (FSM) and the Republic of the Marshall Islands (RMI) because non-availability of disease-free and elite seedlings, shortage of trained agricultural professionals, and limited acquaintance of farmers with commercial cultivation techniques have significantly restricted pineapple production in the Micronesian region. The ultimate aim of this project is to create opportunities of income generation for local farmers through providing a means of sustainable commercial pineapple cultivation, and thereby, improve food self-sufficiency, and nutritional security in the Micronesian region.

This project is of great significance as it is specifically designed to optimize micropropagation protocol and nursery management systems to produce elite and disease-free pineapple seedlings in bulk quantities in the Micronesian region. The project utilized biotechnological techniques for micropropagation and greenhouse acclimatization of tissue-cultured plants. The project activities also include educating local farmers in sustainable agriculture practices, and providing appropriate recommendations and training for commercial pineapple production.

The pineapple (Ananas comosus L. Merrill), a tropical plant with edible multiple fruit consisting of coalesced berries, is the most economically significant plant in the Bromeliaceae family. The project involved undertaking a research study to develop an economically feasible, efficient, rapid and reproducible multiplication protocol for two local commercially important pineapple cultivars (Ananas comosus cv. Kosraean and Ananas comosus cv. Hawaiian). A highly efficient in vitro multiplication method for both cultivars was developed by using apical and lateral meristems. Murashige and Skoog, 1962 medium (MS) was used throughout the study. The cultures were initiated on MS medium augmented with various combinations and concentrations of 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA). The medium augmented with 4.5µM BAP and 2µM NAA proved best for culture establishment. For further growth and subsequent multiplication, established cultures were first transferred on MS medium augmented with 9µM BAP and 3.5µM NAA, and later on the MS medium without any growth regulators. The number of multiple shoots produced from each explant after two subcultures varied from 18 to 35. A 16-h photoperiod with a temperature of 25^oC day and night, light intensity of 80µmol m^-2 s^-1, and 60% relative humidity were maintained for multiplication. Many micropropagated shoots formed roots while they were still on the multiplication medium. However, maximum rooting was observed on MS medium augmented with 0.5µM NAA and 0.5µM BAP. Rooted plants were transferred to soil: compost (10:1, v/v) mixture in pots and acclimatized with 93% survival rate. Hundreds of fully acclimatized, tissue-culture produced pineapple plants are ready to be planted in the field.

Successful implementation of this project has resulted in the development of a high efficiency micropropagation method for mass production of elite, diseases-free seedlings, and acclimatization of hundreds of tissue-culture raised plants of selected pineapple varieties through appropriate nursery management systems. Preparation has been started for designing, implementing and conducting research trials for commercial pineapple cultivation at multiple sites as well as for collecting data for analysis, and the development and publication of a commercial pineapple cultivation guide to specifically suit the needs of local farmers. Recommendations will be provided for sustainable pineapple production through training workshops, hands-on trainings, farm visits, and field days. Three pilot sites in the Micronesian region, specifically in the Federated States of Micronesia (FSM) and Republic of Marshall Islands (RMI) will be developed as demonstration sites to carry out research, extension, and education activities of the project to encourage and promote sustainable commercial pineapple production among local farmers in the region.

KOSRAE, FSM. Dr. Virendra M. Verma, Researcher and Extension Specialist, Kosrae Agricultural Experiment Station.

The United States Department of Agriculture, National Institute of Food and Agriculture (USDA-NIFA) has approved another multi-state/site research project for Micronesian region. The project entitled: Pineapple Micropropagation and Commercial Cultivation to Enhance Productivity in Micronesia, is the second multi-state/site research project initiated after recent recommendations from the College of Micronesia’s Board of Regents. The project director of this project Dr. Virendra M. Verma shared that this project aims to enhance pineapple production in the Federated States of Micronesia (FSM) and the Republic of the Marshall Islands (RMI) through micropropagation and commercial cultivation. Non-availability of disease-free and elite seedlings, shortage of trained agricultural professionals, and limited acquaintance of farmers with commercial cultivation techniques have significantly restricted pineapple production in Micronesia. This project aims to enhance pineapple production to improve food self-sufficiency, nutritional security, and provide means of income generation in Micronesia.

This project is of great significance as it is specifically designed to optimize micropropagation and nursery management systems to produce elite and disease-free pineapple seedlings in bulk quantities, provide appropriate recommendations and adequate opportunities to educate and train local farmers in sustainable and commercial pineapple production. Pineapple (Ananas comosus L. Merrill), the most economically significant plant in the Bromeliaceae family, is one of the most important horticultural crop in the tropical and sub-tropical regions of the world. In Micronesia, pineapple is a desirable fruit in traditional feasts. The crop also has great potential for export. Mainly grown for fresh and canned fruit and juice, pineapple is the only source of bromelain enzyme that is used in pharmaceuticals and as a meat-tenderizing agent. The fresh fruit is very low in saturated fat, cholesterol and sodium. It is also a good source of dietary fiber, thiamin, vitamin B6 and copper, and a very good source of vitamin C and manganese.

To overcome the limitations affecting pineapple production in Micronesia, the project activities include: Collection of pineapple germplasm of local cultivars and establish in vitro cultures of collected germplasm; refinement of micropropagation protocol for multiplication of collected pineapple germplasm; optimization of acclimatization and nursery management systems to produce elite seedlings for commercial pineapple cultivation; designing, implementing and conducting research trials for commercial pineapple cultivation at multiple sites and collection of data for analysis; and development and publication of commercial pineapple cultivation guide to specifically suit the needs of local farmers. Recommendations will be provided for sustainable pineapple production through training workshops, hands-on trainings, farm visits, and field days. Two pilot sites in Micronesia i.e., one each at the Federated States of Micronesia (FSM) and Republic of Marshall Islands (RMI) will be developed as demonstration sites for research, extension, and education activities of the project aiming to promote commercial pineapple production.

The extension and outreach activities will develop positive attitudes, zeal for learning sustainable cultivation techniques and farming aspects of pineapple, and will change the behavior of the participating farmers. Participants of extension and outreach activities will be guided to adopt recommended techniques and start commercial pineapple production farms. The ultimate goal of the project is to develop sustainable commercial pineapple production systems in Micronesia through providing elite, disease-free pineapple seedlings and by providing training to local farmers to improve their knowledge and skills for sustainable, commercial pineapple production.